Sentence examples similar to in the subsequent template from inspiring English sources

Here it should be noted that an intact aa-binding site would still be important for a proto-tRNA to charge its cognate amino acid, while some residues in the site would no longer be necessary in the subsequent template-recognition. Then how could this seemingly contradictory situation be settled?

Here it should be noted that, without a proteinacious AARS, an intact aa-binding site would still be important for a proto-tRNA to charge its cognate amino acid, while some residues in the site would no longer be necessary in the subsequent template-recognition, due to the degeneration of the recognition loop.

Using the primers designed by our new scheme, the newly synthesized PCR products can be used as the templates in the subsequent PCR amplification cycles, which significantly increased the PCR efficiency and required less template DNA while it is unfeasible in the QuickChange™ or the modification reported previously [ 13].

The PCR products were pooled and used as a template in the subsequent overlapping PCR.

Following RNase H-mediated second-strand cDNA synthesis, the double-stranded cDNA was purified and served as a template in the subsequent in vitro transcription (IVT) reaction.

Amplification by ePCR of the single DNA molecule attached to the bead increased the number of copies of template in the subsequent eCFPS reaction, thereby increasing hydrogenase production and eventual signal generation.

For amplification, 4 µg of total RNA were first reverse-transcribed using T7-oligo (dT) promoter primer, followed by RNase-H-mediated second-stranded cDNA synthesis (Invitrogen Corp .. Double-stranded cDNA was purified and served as a template in the subsequent RNA polymerase amplification (MEGAscript T7, Ambion Inc., Austin, TX).

The cDNA was used as a template in the subsequent PCR or stored at –20°C.

The double-stranded cDNA was purified and served as a template in the subsequent in vitro transcription reactions.

After RNase H-mediated second-strand cDNA synthesis, we purified the double-stranded cDNA, which served as a template in the subsequent in vitro transcription (IVT) reaction.

Following RNase H-mediated second-strand cDNA synthesis, the double-stranded cDNA were purified and served as a template in the subsequent in vitro transcription reaction (Affymetrix, Inc).