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At the beginning of each block, a 100% valid cue would inform participants to maintain the orientation of a bar presented in a specific colour in the subsequent sequence of four oriented bars.
Technically, the foundations resulting in the underrepresentation of these regions in the subsequent sequence reads have many sources: for example GC bias affects the stability of the DNA strand which could influence the read ability or modify the probability of a fragmentation.
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The BigDye Terminator v 3.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) was used in the subsequent sequencing reactions according to manufacturer's instructions.
Among virologically failing patients E157Q was noted in one patient with N155H mutant, in three E157Q variant was present at baseline and consistently in the sequences obtained on RAL therapy while in two patients either R263K or L74IL were present at baseline and disappeared in the subsequent sequences on virologically unsuccessful treatment.
Similar to the previous analysis, Figure 2 includes different plot and sequence traces for MUTYH exon 13; First round: mutations clearly distinct from the wild type controls; but R426C heterozygote and G396C heterozygote have identical heteroduplex melting patterns (this differed in the subsequent sequencing).
Another way is a recently demonstrated path dependency approach, which asserts that previous activities in the landscape will influence the subsequent sequence of events.
A frameshift mutation is an insertion or deletion in the coding sequence which changes the reading frame resulting in a different translation of the subsequent sequence.
However, one drawback of second-generation sequencing technology is its relatively short read lengths, which has resulted in difficulties in subsequent sequence splicing, assembly, annotation and bioinformatics analysis [8].
There are many possible variations in the subsequent DNA sequence detection methods used, which achieve diverse levels of performance and adequacy.
This allowed us to use IRDye® labeled M13F and T7 sequencing primers (LI-COR Biosciences) in the subsequent cycle sequencing reactions.
To investigate which quasispecies would become dominant in the subsequent passages, sequencing analysis of bacterial clones containing the PCR fragments from p1, p3 and p5 was performed.
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