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In the subsequent replication phase it is possible for that main effect to be confirmed (Figure 3B), no effect to be detected (Figure 3C), or a main effect in the opposite direction may be detected (Figure 3D).
To analyze the replication of plasmids in the subsequent replication rounds, COS-1 cells were transfected using Turbofect (Fermentas, Glen Burnie, MD) according to manufacturer's protocol.
The excessive number of changes results in loss of genetic information and production of largely defective virions in the subsequent replication cycle.
Only those SNPs that satisfied a concordance rate of greater than 99% in duplicates and a genotype success rate of greater than 99% were included in the subsequent replication phases.
TO-cells used in this study were not pretreated with IFN prior to SAV-3 infection and concordant with previous findings once infection was established, IFN released through downstream signal transduction had no inhibitory effects in the subsequent replication of the virus [ 6].
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Slippage error creates a loop in one of the strands that gives rise to an insertion or a deletion in the subsequent replications, depending on the strand specificity (replicating or template strand) of loop formation.
Slippage creates a loop in one of strands that gives rise to an insertion or a deletion in the subsequent replications depending upon if the loop is formed in the replicating strand or in the template strand respectively.
A flow chart of the consecutive analysis steps is depicted in Fig. 1, and a description of the population samples investigated in the GWAS and subsequent replication studies is given in Table 1.
EMS is a highly mutagenic substance that preferentially alkylates guanine bases leading to the DNA-polymerase favoring the placement of a thymine residue instead of a cytosine residue opposite to the O-6-ethyl guanine in the subsequent DNA-replication step.
The various dinucleotide repeat sequences had very different effects on pausing in the first and subsequent replication cycles.
However, the probability of replicating the specific QTL detected in the initial GWA will generally be low in subsequent replication studies.
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