Today, Ms. Petrobelli turns the supple material into deceptively simple objects, including transparent plates whose colors merge in the stacking.
The grain size gives an indication of the number of layers in the stacking.
Another method to introduce band gap is to apply electric field in the stacking direction of BLG [11 14].
Rietveld refinements against X-ray and neutron powder diffraction data revealed a significant relative compression in the stacking axis (c-axis) of the new compound.
Labeling in the stacking region may result form non-specific interactions and/or network like crosslinking of macromolecular complexes.
In all cases, however, massive labeling was detected only around 70 kDa and in the stacking region, even if monomers became completely undetectable (data not shown).
The particles were retained in the stacking region of the gel while all of the captured proteins were electroeluted from particles and resolved in the gel.
The variability in the stacking residue position may reflect different needs for conformational flexibility in this region of the DEAD-domains.
Fibrils, because of their extremely high molecular weight, do not penetrate into the separating part of the polyacrylamide gel; thus, when present, they appear as smears in the stacking portion of the gel.
However, the signal at the lane of Thio-CS-B2 was detected in the stacking gel area, indicating that it was not mobilized.
(H ) A representative blot shows mutant AR retained in the stacking gel of SDS-PAGE gels as high molecular weight aggregates (arrow).
