The school was considered as the primary sampling unit and individual children as the secondary sample unit (in clusters of 30 students per school), selected in two stages utilizing school attendance registers [ 28].
Moreover, in all analyses, the secondary sampled models consistently collapsed nodes e or h in Figure 1 (similar results were obtained for a dataset with only 5 loci; data not shown; see methods and Additional files 2 and 3).
[ 29], p.29. "The second stage of the sample design was the selection of VPs – in this case, households – which formed the secondary sampling units.
Villages and neighborhoods constitute the secondary sampling units (SSU) in this study.
The secondary sampling units were hamlets in the selected wards.
The primary sampling unit was schools and the secondary sampling unit was classes in the school that best corresponded to 10-year-old students.
Table 1, below, describes the participant characteristics in the secondary sub-sample analysis.
Non-specific staining by the secondary antibodies was monitored by omission of the primary antibodies in coverslips stained in parallel and then using the exposure times for stained samples that gave no signal in the secondary-only sample.
In the secondary school student sample, the bMCU measure and the FCI score correlated significantly with the grade in Newton's mechanics (r = .48 and r = .38) and with one another (r = .63).63
Moreover, when we performed the same FSD analysis of the amide-I peaks in these γ-HS samples, we observed changes in the secondary structures of these samples (Table 4).
For example, with the initial validation cohort (n = 4, σ = 0.3) the delta threshold was calculated to be 0.5, whereas by increasing the sample size to 10 in the secondary cohort of B-CLL samples while maintaining the same standard deviation the maximum required delta threshold required to obtain 80% power was lowered to 0.3.
