Proteins which physically interact are often expressed at the same time in the same tissue (Narayanan et al. 2010).
The tumour can expand locally in the same tissue by cellular invasion and systemically to other organs, a process known as metastasis.
If the aim is to compare drugs, the experiment should be conducted in the same tissue and with the same design.
As this technique becomes more sophisticated and microstructurally informative, there is increasing value in comparing diffusion MRI with microscopic imaging in the same tissue samples.
In addition, mosaic phenotypic analysis, unlike classical single gene-function analysis, requires clear labeling and detection of multiple cell clones in the same tissue.
This enzyme appears to be distinct from two other adenylate cyclases present in the same tissue, which are activated by dopamine and by 5-hydroxytryptamine, respectively.
Thus, depletion of targets is expected for genes that are expressed in the same tissue as the miRNA [24], [44].
Interestingly, a similar accumulation has been observed with a HA-Bou tagged version in the same tissue [7].
As expected, miRNAs derived from the same hairpin were expressed at a similar level in the same tissue.
For comparative purposes we also assayed the expression of MT2 and MT3 in the same tissue collection (Fig. 6B).
The localization of Q19207/Q95005, another pair within PPI-Indel1, was also found in the same tissue (data not shown).
