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We use a zero-one functional similarity matrix with all pairs of proteins in the same specificity group receiving a one.
The distance between two sequences is defined as the negative logarithm of the frequency of assigning them to the same group by the SDPgroup procedure: (9) d (s e q 1, s e q 2 ) = = − log (# (s e q 1 and s e q 2 are in the same specificity group ) 10000 ).
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These data also demonstrate that murine VEGF-A and VEGF-C are active in frog with the same specificity toward blood and/or lymphatic endothelial cells as in mouse and humans.
The F. poae-specific primers, designed in this study, showed the same specificity as primers Fp82f/Fp82r developed previously.
We used the de novo motif discovery program MEME to identify motifs overrepresented in TR4 binding sites to determine if TR4 has the same specificity in vivo.
Our method resulted in much higher sensitivity in identifying associated genes with the same specificity compared to the other two algorithms (Table 1).
In order to estimate the approximate concentration of antibodies in an antiserum that have the same specificity as a given MAb isolated from mice immunized with the MCP, we measured the dilution of antiserum required to compete with the binding to gp120 of a biotinylated MAb, as compared with the MAb itself.
The DNA sequence of clone pEnA1 was used to design species-specific primers, the results maintaining the same specificity patterns observed in the initial hybridization assays.
Napsin A showed a higher sensitivity in primary ADC than that of metastatic ADCs (92.0% vs 67.8%, P < 0.05), and the same specificity in both primary and metastatic ADC (100% for both).
Due to the lack of Fc domains in the scFv proteins, the fluorescent fusion proteins showed more than 20-fold reduced background fluorescence compared with whole antibodies of the same specificity in experiments with effector cells expressing the high affinity FcγRI receptor CD64.
We thus wanted to know whether negative selection of self-specificities within a polyclonal repertoire would also be characterized by an increase in selection of nTreg expressing the same specificity.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com