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The mats were first mechanically drawn in 90 vol.% ethanol aqueous solution and then immersed in the same solution for 30 min.
The passive state of SS remains in the same solution for several days even after the PANI films were completely removed.
The next day, the slides were subjected to successive washes in PBSTX 0.8% and placed in a solution of PBSTX 0.8%/NHS 1/2% BSA (or PBSTX 0.8%/NRS and1/2% BSA) for 20 min; were subsequently incubated for 1 h with the secondary antibody i.e., horse anti mouse diluted 1 800 in the solution of PBSTX 0.8% + BSA 5% for HSP70 and cTnT, or with goat anti rabbit diluted 1 2000 in the same solution for WT1.
For the different samples soaked in the same solution for a given duration, the strength of samples containing fly ash is larger than that of samples in absence of fly ash, and the strength of sample containing quartz sand is larger than that of samples in absence of quartz sand.
After perfusion-fixation, brains were removed and post-fixed in the same solution for four hours.
The brains were removed from the skull and postfixed in the same solution for 4 h.
Similar(27)
They were after embedded in a block of the same solution for 1 h at 4°C.
All control and odour treated larvae were processed together in the same solutions for the same amount of time and were compared side-by-side.
Similar experiments for the proposed model in multiple allocation case result to the same solutions for both formulations.
The coverslips were then incubated with the appropriate secondary antibody conjugated to either DyLight 549 or DyLight 633 (Pierce) diluted in the same solution used for primary antibodies and incubated for 1 h.
Rinsing was performed in 0.4 × SSC/0.3% Igepal at 75°C, for 4 min, then in the same solution at 20°C, for 2 min, followed by 5 min in PBS.
More suggestions(17)
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com