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The nine bar-coded samples were multiplexed with three other samples (unpublished data) in the same sequencing lane.
Small studies might sequence the highly expressed genes from many different tissues in the same sequencing runs without the need for bar-coding.
The intensity of wild-type and mutated bands was estimated by comparing them to the neighbouring bands in the same sequencing lane used as a reference.
Sequencing methods were described previously 15, enabling analysis of two tumors and one healthy tissue sample in the same sequencing run.
The intensity of wild type and mutated bands was estimated comparing to the neighbouring bands in the same sequencing lane used as a reference.
The same three or six nucleotides in the regions of frameshift were interrogated for each mutant transcript in the same sequencing direction.
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You were involved in the same sequence of events the interrogation of Abu Zubaydah.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com