We confirmed these finding by measuring EB3 movements in several cells and obtaining essentially the same quantification results (data not shown).
Importantly, we confirmed our observations using an independent RNA polymerase III subunit, Rpo31 GFP, in gpn2 -2 and gpn3 -1 mutants using the same quantification scheme.
Remark that using the intensity values amounts to using the same quantification method as in ArrayGauge.
(F ) The same quantification method used in the breast array was adopted for the lung tissue array.
To test this prediction we did the same quantifications in embryos heterozygous for a Dyrk1a null mutation (Dyrk1a+/− embryos) (Fotaki et al., 2002).
In clinics, lesions of different sizes and locations might be present in the same patient and quantification accuracy in these cases will vary.
The enzymatic reaction was in the same conditions as for the quantification of total GSH, except that the volume of the derivatized sample was 50 μL.
In parallel, in the same subjects we performed mesothelin quantification based on the FDA approved ELISA (Additional file 3: Table S2).
For Library A and B, we loaded the same number of DNA molecules, based on prior quantification, in the same numbers of lanes (Table 1), and confirmed that it resulted in comparable numbers of reads (Additional file 1).
First, the human β2 M gene and HIV-1 DNA (Gag) were simultaneously amplified in the same tubes used for pre-quantification PCR.
