Furthermore, newborn rat brain cells grown in a primary culture showed the same localisation pattern of porphobilinogen deaminase in the nuclei.
Immunofluorescence assays using the antibody showed that ARL6 is present in a punctate distribution throughout the cell body in both bloodstream form (BSF) and insect procyclic form (PCF) T. brucei and has the same localisation in non-dividing and dividing cells (Fig. 1A).
Cohesin assembled along lateral elements of the SC and showed the same localisation pattern as in wild type in the later stages of prophase I, even after Nipbl was removed from chromosomal axes.
However, in a younger gynoecium (stage 10) the same localisation pattern with lower intensity was found, but at least three cell layers deep in the future style and two cell layers deep in the future valve margins, the future replum, and the future valves (data not shown).
Moreover, they showed the same localisation for TbKIN13-1 and TbKIN13-3 (nucleandand cytoplasmic, respectively) in cells containing either tagged or untagged protein (Fig. 3), demonstrating that the localisation of the GFP-tagged proteins was representative of that for the wild-type protein.
In addition, even patients with the same anatomical localisation and stage may experience different outcome and treatment morbidity on the same treatment modality.
Although DLO pyrophosphatase activity has been identified in various microsome preparations [13], [19] we wanted to demonstrate that Man7GlcNAc2-P generation and ER-mediated polypeptide N-glycosylation [41] occur at the same subcellular localisation in permeabilised cells.
Where the strains analysed were more numerous, particularly in lineage IV, the phylogeny was resolved into clusters corresponding to strains with the same regional localisation.
Therefore, we developed in parallel a quantitative real-time PCR approach based on TaqMan methodology, targeting a smaller fragment of the same genome localisation.
One line (CPTI-003917), coding for the microtubule binding-protein Jupiter, showed a perinuclear pattern that could be distinguished from a nuclear envelope localisation (Fig. 3E), consistent with the localisation of a GFP trap in the same protein (Karpova et al., 2006).
