Jefferson Airplane later sued Berkeley Systems in turn for the use of the same flying toaster emblem.
Adult flies of 1 3 days old were monitored individually in autoclavable plastic tubes (5 mm in diameter and 95 mm in length) on the same fly food described above under 12-hour light-dark cycles at 22°C with 45 60% humidity.
Our initial crossing strategy involved the maintenance of both UAS-endoA* and the TM3 GAL4-Kr UAS-GFP Sbalancerer in the same flies.
Conventional breeding strategies and/or recombination experiments are used to combine gene mutations (e.g., fra4), the Gal4 driver and/or UAS transgenes in the same fly.
Combining the Syx5 suppression and over-expression transgenes in the same fly results in a moderate hypopigmentation of the thorax (Figure 3D) similar to that seen in the moderate copper deficiency caused by Ctr1A suppression [Figure 3E, 26].
For each compound we calculated the ratio of its relative abundance in each 6-day-old fly (virgin or mated) and in the same fly at 4 days ("Post/Ante ratio").
As we previously observed that act-GAL4 driving UAS-clb was sufficient to allow (hmgcr11635 /hmgcr11635 ) flies to survive until adulthood, but not to rescue the sexual dimorphism in locomotor activity, we combined these two driver lines (act-GAL4 and DI-3) in the same fly, to drive hmgcr gene expression.
We combined two split-GAL4 drivers for cell types with either similar or opposite effects in the same fly.
In addition, UAS-nuclear red fluorescent protein (RFP) was included in the same fly line to independently label IPC cell bodies with RFP.
The longer PCR product disappeared in the same flies when the Cre recombinase was introduced to remove the sequences between two loxP sites, leaving a clean mutation resembling that from the "ends-out" event (Fig. 5A,D).
