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Membranes were stained with the primary and secondary antibodies diluted in the same blocking solution.
Then the coverslips were incubated with mouse anti-β3-tubulin (TuJ1) antibody (Covance) prepared in the same blocking solution overnight at 4 °C.
Then the coverslips were incubated with mouse anti-β3-tubulin (TuJ1) antibody (Covance) and rabbit anti-tyrosinated tubulin (ABT171) antibody (Abcam) prepared in the same blocking solution overnight at 4 °C.
Sections were then incubated with calbindin antibody (Sigma) diluted 1 500 in the same blocking solution for 48 h at 4 °C, washed in PBS four times (20 min each) and incubated with secondary antibody (Alexa 488 goat anti-mouse IgG, Molecular Probes) using a 1 500 dilution for 48 h at 4 °C.
Cells were incubated with primary and secondary antibodies for 1 2 hours in the same blocking solution.
PVDF membranes were blocked in PBS containing 0.1% Tween 20, 1% BSA, 5% dry milk (BIO-RAD, Herts, UK) for 1 h at room temperature then incubated with the primary antibody in the same blocking buffer.
Similar(30)
We will compare vaccination efforts in areas within a block that have CMCs with non-CMC areas in the same block.
As a child, Ralph lived in the same block as Varèse in Greenwich Village.
The suspects lived in the same block of flats as the 22-year-old.
In the same block, inmates are taught painting and decorating skills and industrial cleaning.
These SNPs showed high linkage disequilibrium in the same block (r2 ≥ 0.99; Supplementary Fig. 2).
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CEO of Professional Science Editing for Scientists @ prosciediting.com