Using the same array as in this study, we previously identified 60 and 119 microRNAs aberrantly expressed in diffuse large B-cell lymphoma (DLBCL) [ 7] and Sézary syndrome (SzS) (T-cell lymphoma) respectively [ 10].
However, using the same array as used in this study for genotyping several other mapping populations, we identified over 5,000 markers segregating in at least one of these populations (data not reported).
After preprocessing steps, such as background correction and normalization, are applied to the raw fluorescent intensities, for each locus, from about 30 replicates in the same array a summarized β-value is generated as follows: max M, 0 max M, 0 + max U, 0 + 100, where M is the average signal from a methylated allele while U is that from unmethylated allele.
Oster et al. [ 59] applied a reduced protein supply to the maternal diet during gestation; subsequently measuring phenotype and transcription in the offspring at different stages post mortem using the same array platform as employed in the present study.
The study by Ladd-Acosta et al. [ 34] demonstrated significant differences in methylation between adult cerebral cortex and cerebellum using the same array platform as we used in our study.
In addition, all of the 45 probes were consistently changed in expression in E2 treated MCF-7 cells according to public available data deposited at the NCBI GEO Databases from three published studies [ 29- 31], which used the same array type as used in our study (Affymetrix HG-U133Plus 2.0) and the cell line (MCF-7).
For example, the current studies randomly selected the fillers in each array so that each participant would not see the same array composition as other participants.
The dataset uses the same array platform as the breast cancer dataset.
Family-owned businesses face the same array of complicated issues as any other form of business.
We also collected from ArrayExpress [ 22] the dataset used to retrain this signature on the custom array currently used in the MammaPrint assay [ 3] as well as the independent validation cohort using the same array [ 19].
