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Interestingly, this GGD /E EF protein contains a EAL site in the reverse orientation.
We also engineered tandem repeats of two identical MC159 DEDs, MC159 DEDs in the reverse orientation, and MC159-caspase-8 MC159-caspase-8 MC159-caspase-89 was replachimeras the corresponding DED of caspase-8.
In KI-MULEs, the ULP protease domain is found in the reverse orientation with respect to the mudrA gene.
Plasmid pHPI-8234 walsolso constructed, which contains the XhoI fragment of HCV1a (H strain) in the reverse orientation.
The second fragment was inserted into the multi cloning site in the reverse orientation directly beside the "forward" fragment.
The presence of polyT at the 5' end of the cDNA sequences confirms that the sequences are transcribed in the reverse orientation (Fig. S4).
These two SIMs consist of a hydrophobic core, with the more carboxyl terminal one in the reverse orientation, such that position 3 is a non-hydrophobic residue.
Since ID elements tend to integrate in both forward and reverse orientations, we also examined the insertion of an ID1 element in the reverse orientation.
The same procedure was performed for each construct in the reverse orientation, and the minimum of the forward and reverse p-values was recorded.
It is flanked by perfect direct repeats (one orientated in reverse orientation) and is integrated between two asn tRNAs, of which one in the reverse orientation.
A pGK-NeoR-pA (1.7kb EcoRV and Sma1 fragment) from PGKneoKXRO was inserted into the multiple cloning site upstream of the most distal portion of the ncx1 promoter in the reverse orientation.
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