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The total biomass was in the range of the validation data (271 ± 165 tODM∙ha−1).
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The ranges of the validation questions are presented in Table 3.
Validation of the method included evaluation of the following characteristics: accuracy and precision, linearity, and specificity/selectivity. Validation experiments followed the FDA Guidance for Bioanalytical Method Validation [ 18]; all results were in the range of the recommended limits.
Out of 202 compounds in the Vss validation set, 43 compounds (21%) were identified as falling outside the range of the descriptor values of the Kt:p dataset.
The range of the bias correction showed reasonable results when applied to and compared with a validation site in Jakarta.
All in all, the recognition rates for the new set of spectra are in the range of those achieved by cross-validation by using model inherent spectra.
The validation applies to a nominal particle Reynolds number in the range of 20 1000.
Validation using UV-detection showed excellent linearity in the range of 0.04 12 μM (R2 > 0.993).
Cross-validation analyses suggested high prediction accuracy in the range of 87 91%.
The validation results showed good sensitivity, specificity, linearity r2>0.9992) precision(RSD<1.63%), accuracy(recoveries in the range of 95.60%-104.76%) and robustness.
Variations were in the range of 5 10%.
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