Origin Pro 16 (Origin Lab Corporation, MA, USA) was used for the preparation of graphs.
A significant disadvantage of the De Bruijn graph approaches developed so far is that individual read information is lost in the preparation of the graph.
Glucose was used as a standard for the preparation of calibration graph (Thayumanavan and Sadasivam 1984) and distilled water was used as blank in place of sample.
Solutions of different authentic samples (catharanthine, vindoline, vinblastine, and serpentine) were prepared in methanol (0.25 mg ml-1) and used at different concentrations for the preparation of calibration graphs, linear in the range of 0.25 25 μg.
GB would like to thank Zachar I. for the help of the preparation of the graphs.
AM participated in the preparation of the article's structure, graphs, and reference list.
Statistical analyses as well as preparation of graphs were performed using Prism (GraphPad Software Inc), and the graphs display the means ± SEM for each group (n = 4 8).
The software package GraphPad Prism 5.01 (GraphPad Software, San Diego, CA) was used for data analysis and preparation of graphs.
The software package GraphPad Prism 6.02 (GraphPad Software, San Diego, CA) was used for data analysis and preparation of graphs.
Preparation of graphs was performed using Prism (GraphPad Software Inc).
Prism 5.0 software (Graph Pad) was used for statistical analyses and preparation of graphs.
