The midpoint of transition curves is dependent on total protein concentration and ΔG00,un is independent of protein concentration, as expected for this model.
The destabilizing effect of the mutations can be seen by the dramatic shift in the midpoint of the transition Cm from approximately 4 M urea for WT and S134N to below 2.5 M urea for A4V, L38V, G93A and L106V at 10 µM protein.
The relative phase value at the midpoint of the transition was also obtained.
The temperature scan curves were fitted to a Boltzmann sigmoid function, and the Tm values were obtained from the midpoint of the transition as described previously [ 35].
The melting temperature was noted as the maximum value from the first heating scan, and the glass transition temperature was taken as the midpoint of the glass transition.
The peak time of subtropical cyclogenesis (the midpoint of this transition) in the North Atlantic is in the months of September and October, when the difference between the temperature of the air aloft and the sea surface temperature is the greatest, leading to the greatest potential for instability.
As an additional check on data fitting, we observed that the measured rate constant for R20A in 0 M urea is at, or is a little above, the midpoint of the transition (visual inspection of Fig. 2d).
In order to assess how the thermal folding reaction can proceed by way of configurational mesostates, we examined the folding ensemble at the midpoint of its folding transition as comprised by the 310 K replica, by using graph manifold learning.
Tm represents the temperature at the midpoint of the unfolding transition.
These forces are determined by finding the force at the midpoint of the melting transition, averaging over the extensions of 0.44−0.46 nm per base pair to minimize any variations due to subtle changes in the slope of the transition.
The presence of additional sites also shifts the midpoint of the transition between sugar-binding and inactive conformations.
