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The indicated zone in the microscopy image is depicted in Fig. 1b.
Capture experiments were performed at room temperature in the microscopy lab of the Plant Biomechanics Group Freiburg.
Electron microscopy was performed in the Microscopy Core of the Center for Systems Biology/Program in Membrane Biology (Massachusetts General Hospital, Boston).
The introduced micro-manipulator was developed to handle these cell cultures, but can be used also in the microscopy.
A third protocol that has been proposed in the microscopy literature is illumination of the scene by a sequence of three sinusoidal patterns, shifted with respect to one another by 1/3 of the pattern's wavelength [4,5].
The video-encoding step produces a lossy signal which is typically avoided in the microscopy community.
In the microscopy vision, the background was blue or purple, and the positive products were yellow or brown.
In the microscopy data, we see that the coating is easily scratched making it difficult not to damage the coating during mounting into the working electrode holder.
At present the mounting of the sample within the working electrode holder can cause damage to the coating (as seen in the microscopy results).
SOIs were selected based on these profiles, having in mind the consideration discussed in the Microscopy part of the Materials and methods section.
Included in the microscopy systems are one multiphoton system with fluorescence lifetime measurement capabilities, nine confocal microscopes, one total internal reflection fluorescence (TIRF) microscope, seven widefield fluorescence microscopes, one laser capture microdissection microscope system, and two stereoscopes and dissecting scopes.
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