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Green also gets the candidates to play armchair detectives, speculating on-camera about the unsolved killing and trying to fill in the local background relevant to the case.
Furthermore, the variation in the local background signal was less than 38%.
A background-corrected intensity for each spot was formed by subtracting the mean of the pixels in the local background from the mean of the pixels in the spot.
We randomly chose one of the "myc-transformed embryo fibroblast vs. brain" array comparisons and determined the signal-to-noise (S/N) values for each channel using the background-corrected feature signals and the variation in the local background signal.
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The median was chosen in this study as the local background because it is representative of the local data and less affected by outliers [79].
To minimize background effects, square outlines were utilized during spot finding to reduce the variance in the contribution of the local background signal.
In a first step, the local background of each spot was subtracted from the spot's signal intensity.
The signal intensities of Cy5 and Cy3 dyes in each spot and the local background were measured using the GenePix 4000B microarray scanner (Axon Instruments, USA) to calculate the net intensity of each spot for analysis.
Figure 2 2D illustration of the determination of the local background in spill-out region.
The small Amazon river fish Microphilypnus amazonicus and the shrimps it associates with, Pseudopalaemon gouldingi, are so transparent as to be "almost invisible"; further, these species appear to select whether to be transparent or more conventionally mottled (disruptively patterned) according to the local background in the environment.
After quantification of the signal intensities in each array were completed, the local background was subtracted for each subgrid.
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