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Taken together, our data demonstrate that calcium plays an important role in the internalization process of ligands via surface nucleolin.
Previously we reported that phosphoglycerate kinase (PGK), the well-known glycolytic enzyme, bound to leafhopper actin and was unexpectedly implicated in the internalization process of S. citri into Circulifer haematoceps cells.
The fact that PGK-FL5, the minimal actin-binding region, conserved the inhibitory activity on the internalization process reinforces the hypothesis that the actin-binding efficiency of PGK is directly related to the relevance of such an interaction in the internalization process.
The number of endosomes incubated with c-RGD-LPAgNPs reached a platform with 49 ± 9 endosomes observed at the first 3 h, while only very few endosomes (2 3) containing PEG-LPAgNPs were found inside cells during the same time period, suggesting the specific ligand receptor interactions played a key role in the internalization process of NPs.
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The participation of PKC activity in the attachment step of the internalization process was studied during L. mexicana proliferation by incubating promastigotes harvested at 2nd (middle log-growth phase), 4th, 6th, and 8th growth days with adherent peritoneal macrophages.
Therefore, in this study we sought to investigate the entry mechanism of wild-type spores by elucidating the cellular components and signaling molecules in epithelial cells required for the internalization process.
The colocalization with the non-resonant image also allows the identification of the volume distribution of MPIOs, which is particularly important in order to determine whether the internalization process has been successful.
All live cell imaging of the internalization process was monitored in 0.75-μm 0.75-μml seconfocaland sectionsed and3 focal plane imagesummarized
In addition, anti-AChR antibodies in SPMG sera are degraded with their targets during the internalization process.
A failure of CRF1 internalization in females could result from sex differences in CRF1 binding to β-arrestin, the protein that initiates the internalization process [ 51, 128, 129].
To directly examine the internalization process, LFnBLA was used to visualize the uptake in CHO PR328 cells.
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