In no event will GNM be liable for loss arising from failure to insert or any errors in the insertion of Inserts 21.
Based on the sequencing results, all the five targeted loci in the four independent clones had indel mutations in the target sites, including both insertion and deletion (Fig. 5A); however, we noted a few cases where 3 or 6 nucleotides were inserted at the genomic loci, resulting in the insertion of 1 or 2 amino acids in the protein, which presumably retained some function.
The open reading frame of human TARDBP was inserted into p3XFLAG-CMV14 vector (Sigma), resulting in the insertion of an 18 amino acid spacer between the TDP-43 C-terminus and 3XFLAG peptides.
This insert contains an alternate stop codon, and results in the insertion of an additional 130 amino acids.
This results in the insertion of 52 amino acids into the protein sequence before ending in a premature stop codon (NM_152906.6 p.R237iNM_152906.6
Because the TargeTron mutation results in the insertion of the group II intron into the selD gene, there are likely polar effects on the downstream selA and selB sequences.
This method consists in the insertion of a variable external source of potential, obtained from a potentiometer arrangement, in series with the cell and the galvano meter or micro-ammeter, M, used for measuring the current, and the connexion of a second galvanometer, G, directly across the terminals of the cell.
This process results in the insertion of repetitive sequences of DNA throughout the genome and is the mechanism responsible for the vast spread of transposable elements in many higher organisms.
We report a case of division of the deep peroneal nerve resulting from a drill used in the insertion of an oblique proximal locking screw in an AIM tibial intramedullary nail (DePuy).
FliD is located at the distal end of the flagellum and plays a key role in the insertion of each flagellin protein at the growing tip of the flagellar filament.
It is rarely painful with difficulties in the insertion of the catheter.
