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These data suggest that IL-6 is not mainly involved in the initial transformation of RGCs into a regenerative state upon IS, but that it may facilitate axon growth in the inhibitory environment of the optic nerve, thereby contributing to enhanced regeneration.
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DH discovered natural competence in T. sp. strain RQ7 and developed the initial transformation protocol.
For the thick-shell samples the initial transformation proceeds via the sliding plane mechanism resulting in stacking faults that considerably decrease the PL intensity.
During the initial transformation assays we used the gfp reporter gene to in vivo identify the transformations events and to evaluate transformation efficiency in the regenerating plantlets.
Most often, the initial transformation happens as "shadow" IT.
Therefore, the cell that underwent the initial transformation to cancer carried a single X chromosome and all X chromosomes in the cancer are descendants from that original copy.
For the initial transformation of miRNA array data, the GenePixPro 6.0 global normalization method was employed in which images and results are normalized together.
In case no colonies could be obtained from the initial transformations, this procedure was repeated for two more times.
Our results demonstrated that in two patients, APOBEC-mediated mutations occurred very early in tumour development and could potentially be important in the initial neoplastic transformation (Fig. 2b).
Such variations in expression levels among independent transgenic lines (from the same initial transformation event) might also arise from the inserted sequence itself.
So far, the transcription factors Stat3 and Stat5a/b have been implicated in bcr/abl-induced initial transformation.
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