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The bioassay showed that the supernatant of B. licheniformis NCIMB-8874 collected at the late exponential growth phase as well as IPTG-induced supernatant from E. coli cells transformed with the comQX cassette resulted in the highest expression lacZ pointing towards a comparably high pheromone bioactivity in both samples.
Interestingly, >90% of the 2,071 genes harboring a TSS-proximal integration were scored as active, compared to 58% of the control dataset, with a significant over-representation (33%) of genes in the highest expression category (red in Figure 1C).
Samples were rank ordered into three equal groups and the percentage of each subtype in the highest expression group is reported for the NKI patient data set.
In addition, the largest increase in H3K4me3 levels was found on nucleosomes of the genes in the highest expression quintile, indicating that the magnitude of the H3K4me3 increase was positively associated with increased transcript levels.
As shown in Figure 4A, the D. melanogaster orthologs of the G. bimaculatus genes in the highest expression category showed markedly lower ENC and higher GC3 than those from the low or moderate categories (P-value ranked analysis of variance [ANOVA] were 2.2 × 10−15 and 2.2 × 10−15, respectively, Dunn's post hoc tests P < 0.05).
Thus, optimization of codon usage for the moderate expression level classes may be mild in this taxon given the relatively low absolute value of the genome-wide R value above, the similarity of Fop to the lowest class, and the fact that ENC showed an effect only in the highest expression category (see Identification of optimal codons).
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In contrast, the highest expression levels in total pancreas were observed for hydrolytic enzymes, including pancreatic proteases, lipases and nucleases (data not shown).
In vitro as well as in vivo, the highest expression of DISC1 mRNA has been found just after mitogen deprivation.
In particular, the highest expression of C3 mRNA was detected in the urine from BXSB mice.
In addition, the highest expression levels of AtPV42a and AtPV42b are detected in dry seeds (Figure 2A).
In contrast, the highest expression of PPARγ protein was observed when cells were treated with Rosi.
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