Sentence examples for in the flow through of from inspiring English sources

At this MW, a clear positive AMC staining was detected in the isolated IC fraction from RA SF, while no citrullinated proteins could be detected in the flow through of RA SF.

The three resulting cell fractions are i) peripheral non-infiltrating NK cells in the supernatant after centrifugation (P), ii) tumor cells in the flow through of the MACS column (F), and iii) infiltrated NK cells recovered after elution from the MACS column (E).

Electrophoretic analysis of the proteins in the flow-through of the C18 phase showed the absence of hemoglobin and larger proteins/peptides, indicating that they had been captured by the C18 phase, preventing these polymerase chain reaction inhibitory proteins from reaching and binding to the subsequent matrix which would be used for DNA capture.

Surprisingly, not all endocanΔ2 is glycanated and 47.4 % (ranging from 42.9 % to 50.2 %) of endocanΔ2 was recovered in the flow-through of DEAE-Sepharose (Fig. 4A).

The digest was loaded on a His-Trap HP column, and P-TEFb lacking the His tag eluted in the flow-through of the column, while undigested protein and TEV-protease eluted later in the imidazole gradient.

In order to maximize the amount of Elba factor recovered for affinity purification, the Elba activity in the flow-through of the S-Sepharose chromatography was recovered and subjected to the pre-purification steps above by using reduced amounts of beads or matrix until the flow through from the S-Sepharose column no longer contained significant amounts of Elba activity.

In both the HEPES/NaCl and BisTris/KCl conditions, the β-lactamase eluted in the flow-through while the majority of impurities bound the column.

(57) The polar metabolites present in the flow-through fraction of the nematode exudate C18 SPE extractions were particularly well suited for semiautomated assignment by COLMAR query as the reference databases contain large numbers of sugars, amino acids, small organic acids, and other common biological metabolites that tend to partition in the aqueous phase.

After 30 minutes incubation, the purified IgG was collected in the flow through by centrifugation of the spin column.

On the other hand, a single polypeptide of 22 kDa was detected in the flow through fraction of the G1485A mutant (Figure 4B, lane 2).

The highest biodegradation occurred in the flow-through cell experiment where the height of the capillary fringe was largest.