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where h i, h ^ i and h ¯ are the observed and estimated heights and the mean of the observed heights, respectively; n is the number of observations used in the fitting; k is the number of parameters in the equation, and ln is the natural logarithm.
In the fitting, k was treated as a global parameter.
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Because of the limitation in the titration study, to estimate the KD value, although we used the same equation to fit the titration data, in the fitting, Kd was the only variable and the maximum polarization change, ΔmPmax, was fixed to the value that was obtained in the titration study of Rox-DprC binds to mC1-CΔ7.
Clusters within the interaction network were obtained using K-means [ 81], K was evaluated from 2-6, results are displayed for the best fitting K value (k = 4).
The derivation of equations used in the fitting is shown in the Appendix —Section 3. The K m, dTTP was fixed at 90 μM in the fitting based on the K m, dTTP for the helicase when translocating on ssDNA substrate (Appendix —Section 4).
The DISCORE program gives a greater range of binding energies using k i coefficients found in the fitting procedure.
The fitting results for k a, k b, k d and α at 125°C and 140°C are shown in Figure 6.
In the fitting intensities fin(x) and fout(x), all points in the local region O k (x) have an equal contribution.
A fitting threshold named confidence interval of fitting error is proposed to strike the balance between fitting accuracy and surface smoothness in the fitting process.
Both Y∞ and Y0 were floated in the fitting process.
At 313 K, the exchange is slightly faster, which allows the fitting of the K D value.
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