Because the rate of pneumococcal carriage has not been reported for any age group in Laos, the first possibility remains to be tested, although children 1 16 years of age living in nearby Vietnam have been reported to have a carriage rate of 44%.
In regard to the first possibility, we did find some evidence of greater comorbidity in this group of patients.
Studies with Xenopus egg extracts demonstrated that eccDNA can be formed from virtually any repetitive DNA [8], arguing in favor of the first possibility.
The steps that could be inhibited by the low pH are illustrated on schematics in figure 2. The first possibility is the inhibition of the proper folding of the CtxB monomers (Fig. 2A, scenario 1).
In order to negate the first possibility we compared our modifiers with genes involved in protein homeostasis (Silva et al., 2011) as well as the ERAD network (Christianson et al., 2012).
In order to test the first possibility, we stained HeLa-eGFP-LIMP1 cells with an antibody against γ-tubulin to mark the centrosomes.
Because of this, primary cough headache should be suspected in old patients and viceversa, secondary cough headache is the first possibility in young patients.
According to the drilled well data, there is no hydrocarbon in the target zone, which excluded the first possibility.
In the second possibility, elongation of the cap is purely a passive secondary consequence of tension that requires ck/MyoVIIA-mediated anchoring.
