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In the ERF subfamily, a shorter conserved motif was observed in ERF-B2, B3, B5 and B6 than in the remaining ERF group (50 amino acids).
The conserved Ala-37 (corresponding to A182 in this paper) in the ERF domain has been suggested to play a major role in the stability of the ERF domain or DNA binding with the DRE element or GCC box [ 56, 59].
GRAMMAR (green line) performs similar to GC in a sample of nuclear families and in the ERF sample, but is more powerful when IPP pedigree is investigated.
Approximately 3,000 individuals were invited to participate in the ERF study.
Of the 2,766 participants in the ERF study, 1,713 (62 %) returned the questionnaire.
Prevalence and heritability of the metabolic syndrome and related traits in the ERF have been reported previously (1, 13).
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Supporting this argument, two recent studies [ 50, 51] have shown that a tomato ERF TF (Sl-ERF.B3), falling in the same ERF IX subfamily, has a strong effect on ethylene signaling and fruit ripening.
In Arabidopsis, the ERF-VII genes are highly expressed, suggesting to be regulated at the post-transcriptional level.
To date, Nakano's classification method has remained a reference for organizing the AP2/ERF superfamily in three families (AP2, ERF, RAV) and the ERF family in ten groups.
On the other hand, in the case of the erf model, the third-order distortion term (C1) is always dominant and higher-order terms become less dominant, and this holds almost regardless of the IBO value.
The study was performed in the Erasmus Rucphen Family Study (ERF), which is an ongoing family-based cohort study in a genetically isolated population [ 23].
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