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It is important to consider possible limitations of the current simulation studies.
In the current simulation study, 3-D accelerations, measured on a frigate were applied to the kinematics of symmetrical and asymmetrical lifting movements and to a pulling task that had been performed under stable conditions.
We acknowledge this limitation as this scenario was not examined in the current simulation study.
Finally, the sample size used in the current simulation study was similar to the baseline sample size in the real life study.
In the current simulation study, the proportion of significant pathway under ORA is expected to be 100%; but, when the structure of the pathway is assembled by the ER model and assessed by degree centrality, there are only 57.4% significant pathways from 1000 simulations.
Wide adoption of the Berger-Exner test into general RCT methodology of future studies could aid the verification of the current simulation results in practice.
Although the simulation framework regarding the introduction of DEGs was the same as that described in the TMM study [ 16], this may weaken the validity of the current simulation framework.
The focus of the current simulations was on studies with a survival or time-to-event outcome, as these occur frequently in the medical literature [ 13].
Finally, in this study fed-batch photoproduction processes are proposed containing only one photobioreactor based on the current simulation.
In the current simulations, a click is defined as 1.0 μm.
In the current simulations a tick is designed to mimic 10 min of biological time.
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