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Furthermore, we tested if our pooled lentiviral shRNA library is able to downregulate the HSV-TK expression in the corresponding selection cell lines.
Eight of the antigens reacted only with the patient serum used in the corresponding selection.
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The lentiviral shRNA-libraries were stably transduced into the corresponding selection cell lines and treated with ganciclovir in order to select for potent shRNAs.
The predictors considered for inclusion in this study, their prediction features and methodologies, and the corresponding selection constraints are listed in Additional file 1: Table S1 followed by a brief description of the six selected methods.
For a given alignment, we define the amount of conserved sequence information as the number of bases in the reference sequence multiplied by the corresponding selection coefficient, which can be computed form the observed sequence identity by solving equation 1 for s (see Methods for more details).
A small red text box will appear inside the label track at the corresponding selection point in the audio track.
It represents the average fraction of beneficial and deleterious mutations and the corresponding selection coefficients acting in the first 50 generations of evolution as a function of the mutation rate μ.
Together, the correlation between HLA frequencies across populations and the corresponding selection for evasive substitutions in the circulating virus argue strongly for the immune selection of HIV and viral adaptation to antigen-processing preferences.
Clicking on the example links will show the corresponding selections.
They were both significant in the corresponding forward/backward selection models.
Assortment was lower than in the corresponding no selection scenario, which resulted in frequency-dependent effects that act against the evolution of cooperation at small frequencies of cooperator.
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