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Both groups also spent significantly more time in the Correct compared with Incorrect corners (Sham: F 1, 20) = 60.6, p < 0.001; Fornix: F 1, 20) = 14.04, p = 0.001), suggesting that while the Fornix group could discriminate between these mirror-imaged corners, they did not show as strong a preference for the Correct corner as the Sham rats.
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The performance level was lower in the scrambled condition (79% correct) compared with the other conditions (85 90%).
In addition, the boundary group spent a greater proportion of time in the correct zone compared to the boundary + landmark group.
Only ~60% of the well-annotated transcripts have probes that had a higher expression levels in the correct direction compared to the erroneous direction (Additional file 3).
The simple effects revealed that the Sham3 group spent significantly more time in the correct corner compared with the ATNx3 group, F 1, 84) = 4.07, p =.047.
The boundary group spent the least amount of time in the correct zone, compared the other two groups, which spent approximately the same proportion of time in the correct zone.
Both the landmark and the boundary + landmark groups exhibited a strong tendency to search in the correct zone compared to the boundary group, which spent little time in the correct zone.
The ATNx2 and the Sham2 rats spent a similar amount of time in the incorrect corners, but the Sham2 rats spent more time in the correct corners compared with the ATNx2 group.
Examination of the simple effects indicated that the Sham1 group spent significantly more time in the correct corners compared with the incorrect corners, F 1, 20) = 16.3, p =.001, whereas the ATNx1 group did not (p >.1).
Post-hoc analysis showed that WAG/Rij rats spent less time in the correct quadrant compared to Wistar rats only at 13 months of age (p < .05) and not at 5 months of age (p = .99).99
The Sham group spent significantly more time in the Correct corner compared with the Fornix group (simple effects F 1, 40) = 16.15, p < 0.001), although the two groups did not differ in the amount of time spent in the Incorrect corner (F < 1).
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