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from the mean of technical replicates (n=3).
In panels B and D, the number of technical replicates is indicated in each bar.
The use of technical replicates of biological replicates results in marginal improvements and is likely unnecessary.
The average correlation among biological replicates, rather than among technical replicates, is used due the small number of technical replicates, if any at all, observed in a typical LC-MS experiment.
Samples were run in duplicate (99.63% of technical replicates within 0.5 cycles).
This paper explores the concept of technological paradigms in the context of complex technical systems.
Additional file 8 (.pdf) shows unsupervised clustering of technical replicates used in the kinome profiling experiment.
All normalizations, filtering, merging of technical replicates and analyses were performed in the BioArray Software Environment database [ 32].
Different number of technical replicates was used in each analytical determination (see specific technique for details).
The MLP may be a useful promoter in the context of a tissue-specific replicating adenovirus.
In the context of RNA sequencing, multiple nested levels of technical replication exist depending upon whether it is the sequence data generation, library preparation or RNA extraction technical processes that are being replicated from the same biological sample.
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