Searching for a possible effect of the mutations on late events, we quantified virion release from the cells by measuring capsid levels in the context of assembled virions.
Because both mutant proteins are expressed (Figure 7G) and likely coassemble to form antiparallel dimers, the results suggest that only a single intact prong surface is necessary for Syndapin activity in the context of the assembled dimer, and so oligomerization is an integral part of PACSIN function.
The WD40 is thus clearly not undergoing tethered diffusion or 'fly-casting' in the context of the assembled complex.
We then mapped F. grandis reads to the F. heteroclitus CYP1A sequence and analyzed its expression in the context of the assembled sequences.
Complex formation can result in the generation of a continuous binding site that spans multiple components and is only functional in the context of the assembled complex.
Importantly, at least three lines of evidence suggest that interaction with AP-3 occurs in vivo in the context of dysbindin assembled into BLOC-1.
Second, half of the reported O-GlcNAc sites are buried in histone-histone or histone-DNA interfaces and therefore inaccessible to Ogt in the context of the assembled nucleosome (Fig. 1).
Neither sLSU- γ- nor asLSU- γ-derived fragments were detected in the 60S fraction (data not shown), which suggests that fragmentation of sLSU- γ and asLSU- γ rRNAs occurs in the context of the assembling ribosome.
This may occur at the level of direct binding, such that there are some E2s that only bind the RING domain of Roc1a and not Roc1b, or at the level of stimulating ubiquitin transfer to substrate in the context of a fully assembled CDL.
First, the results from different modelling techniques are critically discussed in the context of middle scale timber-to-timber samples assembled with two screws.
