Exact(9)
The sequences in the complete alignment were first divided into more related subfamilies using the Secator program [61].
Relative Entropy (RE) is calculated as the deviation of the amino acid distribution (for a particular column) of one subfamily from that of another subfamily in the complete alignment.
The sites showing significant signal for positive selection (Table 3, test 6) are moderately conserved in the pfam alignment for the matK amino terminal region (positions 224 and 277 in the complete alignment of pfam01824), but no function has been hypothesized for this portion of the matK protein.
Private mutations in each time point in each subject were calculated using the InSites function within DIVEIN [48], in which a private site is defined as a site where only one sequence in the complete alignment has a base that is different from the consensus state.
Finally, alignments of the submatrices are joined in the complete alignment.
Each reflects the relative importance of the one-to-i mappings in the complete alignment.
Similar(51)
Both new families possess several family-specific inserts in the region between the highly conserved structural elements located in loops T1 and T4 (see also the complete alignment in Additional file 2).
To some extent, sequences from the same population cluster together, but there is little intraspecific structure in the phylogeny of the complete alignment.
Afterwards, the ExpaRNA output is used as anchor constraints for LocARNA in order to obtain the complete alignment of the two RNAs.
The complete alignment in clustalw format is shown in Supplementary Data S1.
The nucleotides in positions 505-992 and 1090-1626, relatove thethe complete alignment, were included in a single phylogenetic analysis which used the sequence data produced by the two nested PCRs.
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