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This observation was consistent with a decrease in P. aeruginosa lung loads and a decrease in the lung permeability index in the CaPa group in comparison to the Pa group.
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No further C. albicans systemic dissemination was evidenced in the CaPa-group.
Administration from T0 (the CaPaCasp0-group) significantly abolished the decrease of positive specimens observed in the CaPa-group (P < 0.001).
Also, in blood cultures at Day 4, P. aeruginosa detection was negative in the CaPa-group whereas they were still positive in 25% of the cases in the Pa-group (Table 2).
It was also specific to Ilp7 neurons, given that GCaMP3 fluorescence intensity was unaffected by yeast in the Capa-expressing Va neurons, used as a control population of six unrelated peptidergic efferent neurons (Suska et al., 2011).
Conversely, delayed administration from day 1 (CaPaCasp1-group) resulted in collecting a roughly similar amount of live P. aeruginosa in lungs than in the CaPa-group creating a significant difference with the CaPaCasp0-group (P < 0.01).
Regarding lung endothelial permeability at Day 4, the efflux of the protein tracer in the CaPa-group was statistically greater than both the control- and Ca-groups (P < 0.01) but significantly lower than in the Pa-group (P < 0.001).
In this study, the capA gene was cloned and overexpressed in Escherichia coli as a C-terminal His6-tagged folding reporter GFP fusion protein.
Several peptide families were initially included in preliminary experiments; the CAPA peptides met the aforementioned criteria best and were thus used for this phylogenetic study.
Early in the development of the CaPA system, weather radar reflectivity was identified as a very promising additional data source for the precipitation analysis, but necessary quality control procedures and bias-correction algorithms were lacking for the radar data.
In lung cultures at Day 4, the Pa-group showed a significant higher amount of live bacteria in lungs in comparison to the CaPa-group (P < 0.001) indicating that previous C. albicans airway colonization promoted the clearance of P. aeruginosa from the lungs.
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