This knowledge has been used to try to predict putative prions in biological sequence databases, though the available methodologies to carry out the task are just a few.
The available methodologies of in silico screening based on a single target seem not effective in studying ligands' effects on biological process comprehensively for some cases[27], [28].
The available methodologies for detecting mRNAs in embryos rely on enzymatic activities and chemical reactions that generate diffusible products, which are not fixed to the detected RNA, thereby reducing the spatial resolution of the technique.
In particular, the available methodology forecasts the use of colony formation assays followed by capillary electrophoresis sequencing [ 5, 6].
Given the complex nature of chemicals with endocrine-disrupting properties as well as the likelihood of a large number of yet unknown EDCs that are present in sewage effluent it would not only be prohibitively expensive but also virtually impossible to analytically identify all of these chemicals due to detection limits in the available analytical methodologies.
Again, the challenge here resides in adapting available methodologies to epidemic models for which standards do not yet exist.
Each referral center from Albania, Algeria, Bosnia Herzegovina, Croatia, Egypt, France, Greece, Italy - Messina and Naples - Malta, Montenegro, Morocco, Slovenia, Spain, Tunisia and Turkey provided a list of 50 unselected consecutive CD cases diagnosed in the center by the best locally available methodologies.
In this paper, we review available methodologies in plant foliar VOC research to provide a comprehensive resource for plant scientists with no background in physics or analytical sciences to move into the field of plant foliar VOC research and select the most appropriate analytical method and sampling approach.
Consider a parameter investigated by the best available methodology found in 80% of the subjects of a cohort and in 20% of a perfectly matched control group [think of, e.g. the dexamethason suppression test (DST) or with a corticotrophin-releasing hormone infusion (Dex-CRH)] of melancholic/ major depression (Carroll et al. 1981; Holsboer 2000).
Current data with regard to initiation, tempo, duration, and progression of puberty in children born SGA are limited and difficult to compare due to the differences in methodologies in the available literature (eg, SGA with or without catch-up, differences in the definition of SGA).
