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If the sequence length (not counting gaps) of the prediction in base pairs is called l, its start position in the alignment in alignment coordinates (i.e. including gaps) is called s and its end position in the alignment (in alignment coordinates) is called e, then the beginning is the region in the alignment from s to s + l (in alignment coordinates), and the ending is the region from e - l to e.
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SR 605 was designated in 1937, following the alignment from US 62, to a junction with the US 36/SR 3 concurrency in Condit.
The process is illustrated in Figure 2B in a 51 bp subsection of the alignment from position 1900 to 1950.
Given DNA sequence alignments from multiple species, PhastCons outputs a probability score for each base pair in the alignment indicating from which state the sequence arises.
The region in the alignment ranging from position 1 to 180 can distinguish the genera (see Additional file 2).
Sites in the alignment missing from greater than 30% of taxa were removed with Trimal ver. 1.2 (Capella-Gutierrez et al. 2009).
Relative orthology is defined as the ratio of the size of the putative retrocopy to the size of the genomic insertion – defined by the break in the alignment "net" (from by the UCSC Browser).
For comparison, we also counted the number of unique amplicon lengths for each locus in the alignment (measuring from the outside of each primer pair) and conducted a Spearman's rank correlation test between the mean OV of the 1-kb segments and the amplicon lengths.
The genome-based transcript sequences derived from human and chimpanzee for each putative orthologous pair were aligned using clustalw (version 1.81 [ 42]), with gap extension penalties set to zero to allow gaps in the alignment arising from sequences missing in the chimpanzee assembly.
The means (H m, K m and M m ) and standard deviations (H d, K d and M d ) of the three biochemical features represent new descriptors of evolutionary information, which indicate how well the biochemical properties of an amino acid position are conserved in the sequence alignment from the PSI-BLAST search.
In cases where the alignments from multiple CRMs overlapped, we selected the one with the lowest E value.
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