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Our future study will focus on complex services, which are decomposed into plenty of atomic services, and make sure the atomic services of the complex service are executed in the accurate sequence.
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Paired-end amplicon sequencing resulted in highly accurate sequence reads due to the overlap of related single sequence reads which is of great advantage over single-read-based clustering approaches.
Empirical Bayes may overlook the best guess in terms of most likely substitution resulting in slightly less accurate sequence reconstruction but may better preserve structural and functional properties (Williams et al. 2006).
As discussed before, modeling symbol insertions in pair-HMMs can lead to subtle issues with potentially negative effects, and considering that our ultimate goal lies in finding an accurate sequence alignment through effective estimation of the symbol alignment probabilities, a method that can directly estimate these probabilities without explicitly modeling insertions/deletions would be desirable.
Applications, e.g. in metagenomics, often depend on accurate sequence information, and access to flow values is important to estimate the probability of errors.
And although LHP is not routinely used in forensic match comparisons of Sanger-based CR haplotypes [14,15], accurate sequence alignment in LHP regions will likely still be desirable as the field transitions to MPS for routine mtDNA data generation.
Further, recent developments in computational resources suggest that merging more accurate sequence and structure searches starting from differing sets of queries will not be limiting in the near future.
Database URL: http://www.bioinf.wits.ac.za/software/fire/evodb Hypothesis testing in molecular evolution and phylogenetics depends upon accurate sequence alignments.
At the same time, he says, no company is sequencing whole genomes to the accuracy the contest required as Nature noted in May, the focus is on less accurate sequences and the subset of the genome that codes for proteins.
See also Fig. 1 for the accurate copolymer sequence structures.
This will require further reductions in sequencing cost and the development of accurate sequence-based mosaicism detection algorithms.
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