The fact that Chk1 retains a basal level of kinase activity in the absence of checkpoint induction may explain why overexpression of Chk1 leads to a sustained G2 arrest, which is independent of Chk1 phosphorylation and the checkpoint pathway, and from which cells cannot recover.
Also, in the absence of checkpoint machinery, these telomeres are stable and are not recruited into fusions [16].
We also substituted a gel in which the +HU lanes are more equally loaded to compensate for the ∼2-fold reduction in DNA due to the lack of DNA replication in cells treated with HU. Figure 4 was intended to refer to the decrease in DSB formation that occurs in certain checkpoint mutants in the absence of checkpoint activation.
These results indicate the absence of checkpoints on daughter maturation before entering the next round of cell division.
The evidence presented before this court clearly established that there is no reasonable or effective alternative method of detection and apprehension avilable to the Border Patrol in the absence of the checkpoints, for even a geometric increase in its personnel or line watch would not leave any control over those admitted as temporary visitors from Mexico.
However, as opposed to a typical differentiated cell, the more flexible cancer cell, in the absence of normal checkpoints, utilizes an invigorated form of reverse evolution for coping with epigenetic stresses.
Therefore, the two mutations SA and SE affect the spindle, and, in the absence of the checkpoint, give rise to small colonies.
The slightly enhanced Rpb1 sumoylation in mec1 sml1 cells is presumably due to the persistence (slower repair) of DNA damage in the absence of the checkpoint activation.
The role of Mik1 in providing resistance to replicative stress in the absence of the checkpoint is presumably to prevent premature mitosis by inhibiting Cdc2 via tyrosine phosphorylation (Figure 5 and[13]).
The role of Mrc1 in providing resistance to replicative stress in the absence of the checkpoint is presumably to stabilize stalled forks and allow them to recover (Figure 6 and [26]).
