Exact(2)
The samples were analyzed before and after immersion in the above mentioned solution as well as in 0.01 M NaOH solution (pH=12), saline phosphate buffer at pH 7.4 (SPB) and esterase/SPB.
Finally, the trachea was clamped at a PEEP of 10 cmH2O, right lungs were removed under maintenance of PEEP and submersed in the above mentioned solution for histomorphologic analyses.
Similar(58)
Solution (45 mL) containing 0.32 M FeCl3.6H2O and 0.16 M FeSO4.4H2O were prepared in deoxygenated milli-Q water and NaOH solution (5 mL, 10g NaOH in 5 mL) was added to the above mentioned solution drop wise so that the final concentration of NaOH in the solution becomes 5 M over a period of 5 minutes at 60°C.
The potential-time curves in the above mentioned solutions show that the anodic films formed on Ti are compact and their thickness depends on the solution type and concentration.
Samples for ultrastructural and cytohistochemical investigations were then transferred into glass vials and fixed for 90 min at room temperature (RT) in the above mentioned solutions.
The above mentioned solutions work either as rigid electronics that works in both modes (transmission and reflection) or as a flexible textile but only in transmission mode.
The sections were then blocked using 10% v/v goat serum (Invitrogen, UK), followed by over night incubation with primary antibody prepared in the above mentioned wash solution at 4°C.
In the above mentioned Example 3.2, by a solution we mean an absolutely continuous function which satisfies the corresponding equation almost everywhere.
Magnetite was precipitated by adding the above mentioned mixed solution to a 200 mL aqueous solution of ammonia maintained at a pH ∼ 10, in an inert atmosphere.
Finally, slices were incubated (1 h, RT) with the following primary antibodies in solution in the above mentioned buffer: mouse anti-CD68 (MRA341 100:100; Serotec, Germany), goat anti-Iba 1 (ab5076, 1 100; Abcam, UK) and rabbit anti-TSPO (NBP1-95674, 1:1,000; Novus Biologicals, UK).
Finally, corrections on the basis of established techniques are also mentioned that solve the problems in the above mentioned paper.
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