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Certain genes correspond to multiple transcripts, and in terms of transcripts, our data consists of 98 pairs with 123 unique transcripts.
It has been found that s.e. in C. persicum resembles that of D. carota in terms of transcripts involved [ 29].
These data contain normalized gene expression in terms of transcripts per million (TPM) and these values were used for the further analysis.
In terms of transcripts not destined for translation, such as ribosomal RNA (rRNA) and miRNA, we would expect that nuclear RNA would be enriched for primary or precursor forms of these transcripts.
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Using solid phase microextraction and gas chromatography mass spectrometry (SPME-GCMS), we have determined that FaNES1 directed to the ER via fusion to the transmembrane domain of squalene synthase or hydroxymethylglutaryl – CoA reductase displayed significant improvements in terms of transcript levels, protein accumulation, and volatile production when compared to its cytosolic form.
With no RNA, target probes behaved like negative control probes both in terms of transcript counts and coefficient of variation (Figure 2B).
The bead-selected liver polyA sample has about 2500 fewer transcripts than either the total or RiboMinus sample and is very similar to the 1× cellulose selected sample in terms of transcript count (Table 4).
While this naïve methodology of duplicate read removal had some positive effect in reducing the discrepancies between AS and SMS in terms of transcript quantification, the drastic effects it has on the number of usable reads in AS suggests a different approach may be desirable.
4 Interpretation of the PCR results in terms of transcript structure.
The only distinguishing feature (in terms of transcript abundance) in the E. ocellatus vgDbEST was the atypically high number of PIV SVMPs.
The maternal care behaviour exhibited post-reproductively by Ceratina mothers is concordant in terms of transcript expression with the alloparental care exhibited by workers.
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