As expected the potential and the daily accessibility indexes present the strongest correlation; we have retained both the indicators in our successive analyses since, how already noticed, they allow us to measure accessibility from different perspectives (overall or 'within-4-h/daily' activities reachable from each zone).
However, to avoid the decrease of the electrochemical signal due to the progressive consumption of ferrocene in multiple successive analyses, it was necessary to regenerate the electrode by reduction of ferricinium moieties, which can be achieved in-situ by square wave amperometry.
All successive analyses in mice with our without treaments were performed starting at 10 AM after the last stress exposure.
Indeed, there was a 26% decrease in dose reduction <85% across successive analyses of a same report (Lyman et al, 2003; Shayne et al, 2006).
Only spots showing at least a two-fold change in their relative volumes were considered for successive analyses.
Logistic regression was used to estimate associations between deprivation (in quintile categories) and the odds of having been at risk of pallor, bleeding, sepsis, or non-diagnosis (each treated as a dichotomous variable), in successive univariate analyses.
To confirm the Hydra origin of the 10'741 genome-unpredicted "RNAseq only" and the 11'582 genome-predicted only sequences, we tested these sequences sequentially in successive BlastN+ analyses, first against the sequences of common Hydra contaminants (Artemia, Curvibacter), then against Hydra genomes.
The heat treatments and successive analyses need several tenth mg of the diatom sample.
Using this reasoning, we chose a grid cell size of 32 km2 for successive analyses.
