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This latter buffer was then used as the basis for LRRK2 elution in subsequent preparations of LRRK2 protein for cryo-EM.
If a mixture appears in the first instance, then very often in subsequent preparations the new form dominates and the old form is no longer obtained.
DNA preparations found initially to have high sheared DNA background were in subsequent preparations treated with lambda exonuclease and RecJf (New England Biolabs) (Balagurumoorthy et al. 2008) and/or Plasmid-safe DNase (Epicentre) prior to electrophoresis.
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This solution was also used as the solvent in all the subsequent preparations of analyte samples.
The railroad may participate in the selection process, as well as in the subsequent preparation of environmental documents.
Subsequent preparations for the trial of Stephen Ward caused a panic in the establishment.
The collection was re-processed in 2004, with subsequent preparation for digitization in 2009.
In order to achieve higher chemical and radiochemical purities, we decided to use HPLC purification for subsequent preparations of 11C MET.
RNA was extracted from 3-mm3 tissue using the AllPrep DNA/RNA/protein mini kit (Qiagen, Valencia, CA, USA) with an on-column RNase-free DNase (Qiagen) treatment to avoid DNA carryover in subsequent RNA preparation.
RNA was extracted from 3-mm tissue using the AllPrep DNA/RNA/protein mini kit (Qiagen, Valencia, CA, USA) with an on-column RNase-free DNase (Qiagen) treatment to avoid DNA carryover in subsequent RNA preparation.
Thereafter, mice were sacrificed by cervical dislocation and tissues were rapidly excised, weighed, and either frozen in liquid nitrogen or stored in 4% neutral-buffered formalin for subsequent preparation of paraffin blocks.
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