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This demonstrates that the property of Scrapie infectivity was not modified by exposure to the RADA in subsequent passage.
Cell-free virus was harvested, titrated and characterized by PRA and PEA prior to use in subsequent passage experiments.
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However, fibroblast cells outgrew their epithelial counterparts in subsequent passages.
Once growth occurred in a second animal generation, no AHGs were lost in subsequent passages because of failure to grow.
Briefly, single cells were plated in ultra-low attachment 24-well plates (Corning) at a density of 20,000 viable cells/ml and 5000 cells/ml in subsequent passages.
In subsequent passages slight variations in the individual expression levels of Krt14 and Krt18 (data not shown) and a significant fluctuation in the number of cytokeratin-expressing cells ranging from 40 to 70% were observed.
Accordingly, when KSHV DNA was analyzed in subsequent passages of unstimulated late-EPCs from patient KS 4, we could observe that the levels of viral genomes may change over time.
In subsequent passages G-2 cells preserved the ability to form multiple cell clusters and three-dimensionally expanding colonies, but acquired a more fibroblastic-like morphology (Figure 2B, C).
In subsequent passages, the mice showed distinct characteristic scrapie phenotypes.
In subsequent passages, hepatocyte-like cells maintained at least 70% of albumin-containing cells.
Long-term growth potential and p16 promoter methylation in subsequent passages were also monitored.
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