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These promoters showed homology to the bacterial −10 and −35 consensus sequences and resulted in strong expression of a bacterial cytotoxin.
The pGL3-Control vector contains the SV40 promoter and enhancer sequences, resulting in strong expression of the reporter gene in many types of mammalian cells.
Transient transfection in HEK 293 cells resulted in strong expression of ActRIIB receptor protein which was not affected by co-transfection of sh-AIIA (see Figure S4D).
Conversely, the infection of 10-DIV hypothalamic neurons with AAV-EGFP resulted in strong expression of EGFP (Figure 4-Fi) in neurons positive for the TUJ staining (Figure 4-Fii).
It has been previously shown that tail vein injection in mice of an AAV vector coding for a reporter gene under the control of the muscle creatine kinase (MCK) promoter results in strong expression in muscles while expression in nonmuscle tissues remains ≤4% of the highest activity seen in most skeletal muscles [22].
PaO2 was lower in strong expression group than in the negative and intermediate expression groups (p = 0.023).
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In arteries with TV in allografts, strong expression of collXVIII and perlecan was observed in the newly-formed neointima (Figure 3A and B).
As mentioned previously, in the strong expression of WRAP53 in biopsies was reduced in the primary tumors after radiotherapy.
In ESCC, strong expression was seen in cytoplasm of cancer cells in most cases, especially at the tumor-stroma interface.
In particular, strong expression was observed in invasive tumour fronts.
In contrast, strong expression of CNGA2 channel was detected in both the endothelium and smooth muscle layers of human arteries [8].
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