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The shorter gap duration stimulus (40-4-20 ms) resulted in strong decrease in the response amplitude for all age groups.
In several countries, lead exposure levels have been in strong decrease over recent years, as documented for instance for Italy (Alimonti et al. 2011).
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Interestingly, mutations in genes involved in generating scaffold RNAs, such as RDM1, DRD1, and NRPE1, also result in strong decreases of 24-nt siRNAs, suggesting that Pol V function contributes to 24-nt siRNA accumulation at the RD29A promoter.
As depicted in Figure 4B, the successful silencing of ASK-1 resulted in a strong decrease in JNK/SAPK activation in irradiated GSH-depleted SQ20B cells.
Centromeric localization of CHD1 was also observed in chicken DT40 cells, and RNAi-mediated depletion of CHD1 in human cells resulted in a strong decrease in CenH3CENP-A centromeric signals [13].
YFP RNAi resulted in a strong decrease in polyQ-YFP levels in Q40Bristol, and a weaker decrease in Q40DR1350.
Cotransfection of the WT RhoB 3'UTR plasmid and pre-miR-21 in HEK-293T cells resulted in a strong decrease in luciferase activity suggesting that RhoB mRNA is a direct target of miR-21.
More importantly, injection of the dimerizer into mice that developed a colon CSC-iCasp9-induced tumor resulted in a strong decrease in tumor size, an increase in tumor cell apoptosis and a clear loss of CD133+ CSCs.
In an in vitro study, it has been shown that a 5 h period of hypoxia resulted in a strong decrease in acetylated H3 and H4 histones in mouse cardiomyocytes, which was completely blocked by TSA [ 94].
Inhibition of SHH signaling by cyclopamine treatment results in a strong decrease in Vegfa expression, that can only in part be rescued by addition of exogenous FGF9 (White et al., 2007).
Addition of already a limited amount of water to AMS reaction mixture resulted in a strong decrease in activity.
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