Sentence examples for in stringent conditions from inspiring English sources

The asymmetric membranes should also demonstrate high oxygen flux in long term operation often encountered in stringent conditions.

The day after the samples were rinsed in stringent conditions (50% formamide, 2× SSC at hybridization temperature), washed for 1 h with a blocking solution (1% BSA in PBS-T) at room temperature followed by an incubation with 10 nm gold-conjugated avidin (BBInternational) and a silver enhancement (Silver enhancing kit, BBInternational).

The membrane with the spotted cDNA was hybridized in stringent conditions.

Detection of artichoke putative targets was performed in stringent conditions, and only genes matching to described Arabidopsis proteins were considered.

Endoplasmic reticulum (ER) stress occurring in stringent conditions is critically involved in cardiomyocytes apoptosis and cardiac contractile dysfunction (CCD).

Human box C/D scaRNAs can be found in WRAP53 immunocomplexes, but only in significant amounts when cells are lysed in stringent conditions (Tycowski et al., 2009).

When I-BAR-expressing cells were extracted in more stringent conditions in the absence of PEG (Fig. 5), the GFP-I-BAR fluorescence (Fig. 5A, insets) and corresponding submembranous material were largely removed exposing the structure of the underlying cytoskeleton (Fig. 5).

In conclusion, we demonstrated that HTs are not negligible in eukaryote genomes, bearing in mind that in our stringent conditions this amount is a floor value, though of a lesser extent than in prokaryote genomes.

This assay was therefore chosen among other cell-based assays in order to test molecules in more stringent conditions and maybe avoid subsequent disappointing results in vivo [25], [26].

Laser manufacturers are nowadays driven by the wide variety of laser applications to build compact and robust devices that are able to perform in more stringent conditions [1].

Whilst the Haber Bosch Process can, to some extent, be viewed as a mature technology, the development of more active (or even more poison tolerant) catalysts which would allow a reduction in the stringent conditions of operation is of significant interest.