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Synthetic solutions of Cr VI) ions and Cd II) ions were taken in stopper bottles and agitated with the prepared adsorbents separately at 30 °C in orbit shaker at fixed speed of 160 rpm.
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Our results fit a model in which the poly ED either by itself or as part of a larger inactivating motif acts as CaV1.2 specific built-in "stopper".
ZnO portions of 0.20 g were soaked overnight in water in stoppered bottles.
These DiI-inserted blocks of brainstem were stored in 4% paraformaldehyde in 0.1 M phosphate buffer (PB, pH 7.4) in stoppered glass jars wrapped in aluminum foil and maintained in a dark cabinet at room temperature for seven years.
Reaction was performed in 10 mM sodium acetate buffer (pH 4.8) in 500 mL shaken flasks with ground in stoppers filled with 200 mL wheat straw suspension.
The leaf segments were washed with distilled water to remove surface contamination and placed in stoppered vials containing 10 mL of distilled water.
Extraction was carried out by macerating the powdered plant materials (100 g) in stoppered flasks containing 500 ml of 95% ethanol at room temperature 25-30°C 25-30°C days.
An excess amount of P60 and caffeine were added together in 2 mL of the selected oil, surfactant, and cosurfactant in stoppered vials and then preliminary mixing was carried out over magnetic stirrer for few minutes.
Saliva including control samples or blank (bi-distilled water), 1.0 ml each, were spiked with [H]testosterone (Radiochemical Center, Amersham, UK, 1200 dpm/sample), and extracted in duplicate with diethyl ether (4 ml) in stoppered glass tubes.
Reactions were performed in 10 mM sodium acetate buffer (pH 4.8) in 500-mL shake flaks with ground-in glass stoppers.
The type I collagen-coated wells were equipped with in-place stoppers to create a migration area on the bottom of each well.
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