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Newport, Bar Harbor & Southampton blaze like three emeralds in stomacher, instead of as they used to, shining solitaire.
Briefly, radishes of each trials were collected, superficially sterilized and then transferred in stomacher bags, added with VIA broth (final ratio 1 10) and homogenized as reported above.
These eggs were placed individually in stomacher bags and gently but vigorously rubbed and shaken in 50 mL of BPW.
For detection of foodborne pathogens with our protocol, 25 g of beef and 225 mL of FPE broth were homogenized in Stomacher bags (Eiken Chemical Co., Tokyo, Japan).
Tissues were homogenized in PBS in stomacher bags, and DNA was extracted (DNAeasy tissue kit; Qiagen, Crawley, UK) and quantified (NanoDrop Technologies, Wilmington, DE).
Samples were mechanically disrupted in PBS in stomacher bags (Seward, Thetford, UK), and serial dilutions were plated on reinforced clostridial agar (Merck, Darmstadt, Germany) containing 0.05% L-cysteine hydrochloride (Sigma-Aldrich) and 50 mg/ml rifampicin (Sigma-Aldrich).
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Selecta enrichment was performed in stomacher-bags for 18 19 h at 41.5 ± 0.5 C°.
Ten g samples of feces were immediately processed in a Stomacher, serially diluted in sterile phosphate-buffered saline, and plated in triplicate onto Sorbitol-MacConkey agar.
Individual eggs were then placed in a stomacher bag (Sarstedt, Australia) and massaged in the bag in 50 mL of BPW for 3 min to detach the bacteria.
For stomaching, samples were placed in sterile stomacher bags (BagPage 400 mL, Interscience).
Ten grams of salad was homogenized in 90-mL sterile saline in a stomacher for 1 to 2 min. Aliquots (200 μL) of decimal dilutions of the homogenate were plated onto vancomycin-imipenem-amphotericin B (VIA) agar (3) and incubated for 24 to 48 h at 30°C.
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