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Recombinant adeno-associated serotype 2-based vectors (rAAV2) possess a number of theoretical advantages for cystic fibrosis (CF) gene therapy because they elicit little or no inflammatory response and generally result in stable expression.
As shown in Fig. 3, transfection with synthetically E6 construct (E6) or along with empty pSilencer4.1-CMV vector (vector + E6) result in stable expression of E6 mRNA, while transfection with E6 construct along with miR-875 (miR-875 + E6) or miR-3144 (miR-875 + E6) individually decreased the expression of E6 mRNA to 53% or 42%.
Will these delivery methods result in stable expression or transient expression of the transgene?
However, inhibition of PHDs activity by DMOG resulted in stable expression of HIF-1α.
Our results show the role of PHD2/3 in stable expression of HIF-α in human ccRCC.
This was preferred as it resulted in stable expression over longer time periods (Suganuma et al, 2002).
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The BrdU phenotype is in contrast to previous reports in which stable expression of AGR2 in H1299 cells had no effect on the cell cycle or DNA synthesis [ 15].
In addition, stable expression of T βRII in these cells decreases tumorigenicity in vivo.
In addition, stable expression of Pygo2 in HeLa cells is reported to exert an anti-apoptotic activity by DNA fragmentation, sub-G1 appearance, loss of mitochondrial membrane potential and the activation of caspase-9 and caspase-3.
The origin of bimodality in the GFP distribution lies in two stable expression states, i.e., bistability.
In vitro, stable expression of hSulf2 or administration of rhSulf2 in breast cancer cells decreased cell proliferation and invasion, corresponding to an inhibition of ERK activation.
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